FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1449906
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT64880.4474772847343207No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT43260.29836416981514663TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT24440.168562651647762RNA PCR Primer, Index 1 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCGCGTATGCCGT24160.16663149197258306No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCGCGTATGCCGTCTT23050.15897582326026655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18760.12938769823698917No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)