FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6218275
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT210700.33883995159429264No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT145940.2346953134108736TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT82930.1333649605396995RNA PCR Primer, Index 1 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCGCGTATGCCGT74050.11908447278385084No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCGCGTATGCCGTCTT72870.11718684040187995No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68950.11088284130245124No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)