FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173130
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT59610.2743048045906135No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT40330.18558484766212788RNA PCR Primer, Index 39 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGT36010.1657056871885253No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTT35480.16326680870449536TruSeq Adapter, Index 11 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27400.12608541596683126No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT25140.11568566997832619RNA PCR Primer, Index 39 (96% over 25bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG22690.10441160906158398RNA PCR Primer, Index 39 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)