FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996560
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT54100.5428674640764228No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT42050.4219515132054267TruSeq Adapter, Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGT30330.3043469535201092No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTT29680.2978245163361965No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT22570.2264790880629365RNA PCR Primer, Index 10 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCT15980.16035160953680663Illumina PCR Primer Index 5 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15170.152223649353777No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG14210.14259051135907522TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTTG11450.11489523962430763Illumina PCR Primer Index 5 (96% over 26bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT10130.10164967488159268TruSeq Adapter, Index 10 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)