FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82730
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT38784.6875377734799955No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT30963.742294210080986TruSeq Adapter, Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGT20462.4731052822434427No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTT20422.4682702768040614No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT17652.1334461501269186RNA PCR Primer, Index 10 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCT11931.4420403722954187Illumina PCR Primer Index 5 (95% over 21bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG10071.2172126193641968TruSeq Adapter, Index 10 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT7600.9186510334824127TruSeq Adapter, Index 10 (96% over 26bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTTG7590.9174422821225674Illumina PCR Primer Index 5 (96% over 26bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCT5190.6273419557597002Illumina PCR Primer Index 5 (95% over 24bp)
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG4120.49800556025625525TruSeq Adapter, Index 10 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC3120.37713042427172727No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTG3080.3722954188323462Illumina PCR Primer Index 5 (95% over 22bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA2930.354164148434667RNA PCR Primer, Index 10 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT2470.2985615858817841No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTC2350.2840565695636408No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTTGA1750.21153148797292395Illumina PCR Primer Index 5 (96% over 26bp)
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC1630.1970264716547806Illumina Paired End PCR Primer 2 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1540.1861477094161731No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCT1400.16922519037833916No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGC1000.12087513598452798Illumina PCR Primer Index 5 (95% over 23bp)
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA970.11724888190499214No Hit
GTCCTAAAGTGTGTATTTCTCATTATCCGTGATTTTCAGTTTTCTCGCCA840.1015351142270035No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)