FastQCFastQC Report
Mon 25 Jul 2022
EGAF00005106273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66614
Sequences flagged as poor quality0
Sequence length101
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT57618.648332182424115No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT45916.891944636262648TruSeq Adapter, Index 10 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTT29184.380460563845437No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGT28114.219833668598192No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT23833.577326087609211RNA PCR Primer, Index 10 (95% over 23bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG16172.4274176599513617TruSeq Adapter, Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCT14452.169213678806257Illumina PCR Primer Index 5 (95% over 21bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTTG12371.8569670039331072Illumina PCR Primer Index 5 (96% over 26bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT10181.5282072837541658TruSeq Adapter, Index 10 (96% over 26bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCT7011.0523313417599904Illumina PCR Primer Index 5 (95% over 24bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA5500.8256522652895788RNA PCR Primer, Index 10 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG5170.7761131293722041TruSeq Adapter, Index 10 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC4810.7220704356441588No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTG4050.607980304440508Illumina PCR Primer Index 5 (95% over 22bp)
CTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTTGA3920.5884648872609362Illumina PCR Primer Index 5 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT3740.5614435403969136No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTC2600.39030834359143723No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC2300.3452727654847329Illumina Paired End PCR Primer 2 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCT2040.3062419311255892No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.24469330771309333No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGC1630.24469330771309333Illumina PCR Primer Index 5 (95% over 23bp)
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC1400.21016603116462007No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT1190.17864112648992703No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTT1110.1666316389948059TruSeq Adapter, Index 10 (96% over 27bp)
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA1080.16212808118413546No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCG1020.1531209655627946RNA PCR Primer, Index 10 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.14411384994145376No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGAGGCT940.14111147806767346No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCGGCTTG870.13060317650944248Illumina PCR Primer Index 5 (96% over 26bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCGGCTTG810.1215960608881016No Hit
CTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTAGACGGGGTACGTGCC810.1215960608881016No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC790.11859368901432131No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG790.11859368901432131No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA780.11709250307743116No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCG770.11559131714054102No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCGTG730.10958657339298046Illumina PCR Primer Index 5 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCGT700.10508301558231004No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTT680.10208064370852973Illumina PCR Primer Index 5 (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.10208064370852973No Hit
GGGTAAATGAGTGCGACGGCCGGCAAGCCCCCGCTCCCCGGGCTCTCGCG680.10208064370852973No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)