FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333671
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT73670.3156828876049794No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT59580.25530591073034714TruSeq Adapter, Index 10 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTT38970.16699011985836906No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGT37250.15961975788360913No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT31060.1330950249628161RNA PCR Primer, Index 10 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26080.11175525598938324No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)