FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2969468
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT91950.3096514257772773No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47170.1588500027614374No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCGCGTATGCCGT45020.15160964859698775No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT42620.14352739278550905RNA PCR Primer, Index 33 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)