FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1632264
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT78280.47957928374331604No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT38890.23825802688780734RNA PCR Primer, Index 33 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCGCGTATGCCGT37010.22674028220925047No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCGCGTATGCCGTCTT26550.16265751128493922No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24480.14997573921865578No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)