FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087892
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT45940.220030537978018No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39710.19019182984560506No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT30910.14804405591860115TruSeq Adapter, Index 1 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGT25030.11988167970373947No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGTCTT23190.1110689633371841No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23000.11015895458194198No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)