FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3326392
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT95950.28845066967453026No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT68740.20665032864436902TruSeq Adapter, Index 1 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGT52210.15695684693806383No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGTCTT51000.15331927205212134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44210.13290676504753499No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)