FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3709099
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT79580.21455345354761357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57480.15497025018744442No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA54470.1468550718112404No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT54340.14650458237971Illumina PCR Primer Index 10 (95% over 21bp)
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT40390.10889437030394712No Hit
GTGTAAGAAATTGCCATTTGAAACAATCCATTTTGTAACTGAACCTTATG38490.10377183245850272No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGT38190.10296301069343254No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)