FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2993000
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT124930.4174072836618777No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT80550.26912796525225524Illumina PCR Primer Index 10 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62510.20885399264951554No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGT62320.20821917808219176No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTT56170.18767123287671234No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT41710.13935850317407283Illumina PCR Primer Index 10 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36970.123521550283996No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)