FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2581018
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT71210.27589888950793834No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT42640.16520613184410182Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGT35320.136845229285499No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33850.13114980213233693No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTT29900.11584576318336409No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT25910.10038674662478139Illumina PCR Primer Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)