FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005106433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1778521
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT56350.3168362926274134No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT37170.20899387749708886Illumina PCR Primer Index 10 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26780.1505745504270121No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGT25840.1452892600087376No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTT22850.12847753835911974No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT19600.11020392787040467Illumina PCR Primer Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)