FastQCFastQC Report
Mon 8 Aug 2022
EGAF00005106439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6378376
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT226750.35549801391451363No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT156910.2460030578316487Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGT109900.17230091170542472No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTT101270.15877082191454375No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT90950.14259115486449842Illumina PCR Primer Index 10 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65450.10261232639781663No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)