FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025975
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT149101.453251784887546No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT113521.1064597090572381Illumina PCR Primer Index 10 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTT79200.7719486342259801No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGT77780.758108141036575No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT59700.5818855235264017Illumina PCR Primer Index 10 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTTCT41340.4029337946831063RNA PCR Primer, Index 12 (95% over 21bp)
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG35610.3470844806159994Illumina PCR Primer Index 10 (96% over 28bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT28090.27378834766929017Illumina PCR Primer Index 10 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24350.23733521771973No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTTCTGCTTG24180.23567825726747726TruSeq Adapter, Index 12 (96% over 26bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTTCTGCT20740.2021491751748337TruSeq Adapter, Index 12 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13640.13294670922780769No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTG12960.12631886741879675Illumina PCR Primer Index 10 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC12030.11725431906235532No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTGA10320.10058724627793074Illumina PCR Primer Index 10 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)