FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785827
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT94841.2068814128300505No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT71500.9098694750880283TruSeq Adapter, Index 11 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTT53550.6814476977757191Illumina PCR Primer Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGT48420.6161661536190535No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT42240.5375228898981583RNA PCR Primer, Index 11 (96% over 25bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTTCT32620.4151040877954053Illumina PCR Primer Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT20580.2618897034589038TruSeq Adapter, Index 11 (96% over 28bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG18510.23554802774656508TruSeq Adapter, Index 11 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17920.22804001389618833No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTTCTGCT16590.2111151691148306Illumina PCR Primer Index 11 (96% over 27bp)
TCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTTCTGCTTG15280.19444483327755346Illumina PCR Primer Index 11 (96% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11980.15245085750425982No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTG9080.11554706061257757TruSeq Adapter, Index 11 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC8000.10180357763222693TruSeq Adapter, Index 11 (95% over 21bp)
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7870.10014926949570326No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)