FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892043
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT31560.3537946040717768TruSeq Adapter, Index 11 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTT26210.29381991675289193Illumina PCR Primer Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT20490.22969744732036462No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT19140.21456364771653383RNA PCR Primer, Index 11 (96% over 25bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTTCT15320.17174059994865717Illumina PCR Primer Index 11 (95% over 24bp)
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG14950.16759281783501467TruSeq Adapter, Index 11 (96% over 30bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT14920.1672565111771518TruSeq Adapter, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGT14700.16479026235282382No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTTCTGCTTG13410.15032907606471885Illumina PCR Primer Index 11 (96% over 29bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTTCTGCT12870.14427555622318655Illumina PCR Primer Index 11 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10370.11625000140127774No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)