FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300838
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT48490.3727597133540072No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT39710.30526476010079656TruSeq Adapter, Index 11 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTT29410.22608503134133534Illumina PCR Primer Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGT25110.19302941642233698No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT24190.18595705230013268RNA PCR Primer, Index 11 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21240.16327936299523846No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTTCT19170.14736654372027877Illumina PCR Primer Index 11 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)