FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080535
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT24860.23007121472233663No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT19620.18157671894015465TruSeq Adapter, Index 11 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTT13240.12253189392291781Illumina PCR Primer Index 11 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12740.11790455653912182No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT12430.11503560736116832RNA PCR Primer, Index 11 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGT11750.10874242851920578No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)