FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2545714
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT123580.48544337659297154No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT97990.3849214797891672TruSeq Adapter, Index 11 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72220.28369251219893515No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTT71540.2810213558946527Illumina PCR Primer Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGT61490.24154323698577293No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT58470.2296801604579305RNA PCR Primer, Index 11 (96% over 25bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTTCT41540.1631762248233698Illumina PCR Primer Index 11 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40410.15873739155301814No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT31220.12263749973484846TruSeq Adapter, Index 11 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30980.1216947386862782No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)