FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3500498
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT144220.41199852135324744No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT96330.2751894159059654TruSeq Adapter, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGT71470.20417094939062957No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTT65860.18814465827433696No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52950.15126419155217344No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT50700.1448365346873502RNA PCR Primer, Index 12 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCT35110.1003000144550861Illumina PCR Primer Index 12 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)