FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887058
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT137690.7296543084526284No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT97900.5187969845123998TruSeq Adapter, Index 12 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTT68010.36040227698353733No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGT66750.3537252167129998No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT50530.26777131386528663RNA PCR Primer, Index 12 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34580.18324820964697427No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCT33780.17900880630060126Illumina PCR Primer Index 12 (95% over 22bp)
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG23100.1224127716265213TruSeq Adapter, Index 12 (96% over 29bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCT23010.12193583875005432TruSeq Adapter, Index 12 (96% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21160.11213221851156668No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)