FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005106487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909243
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT48790.5366002267820593No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT28720.3158671554248974TruSeq Adapter, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGT27720.30486899541706675No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTT19340.21270441455144554No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT16670.1833393273305376RNA PCR Primer, Index 12 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11040.12141968648645082No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCT9620.10580229927533123Illumina PCR Primer Index 12 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)