FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2995809
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT92410.3084642579016219No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT61090.20391820706860816Illumina PCR Primer Index 10 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGT53470.1784826736283922No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTT52540.17537833686994064No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41110.13722503670961667No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT36460.12170335291735888Illumina PCR Primer Index 10 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTTCT31810.1061816691251011RNA PCR Primer, Index 38 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)