FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549893
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT25980.47245555044344995No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT22870.4158990930962933Illumina PCR Primer Index 10 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTT18250.3318827480982664No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGT13930.2533220099182932No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT13830.25150347431227527Illumina PCR Primer Index 10 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTTCT10870.19767482037414552RNA PCR Primer, Index 38 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8290.15075660173888375No Hit
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCT7820.14220948439059963Illumina PCR Primer Index 10 (96% over 27bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTTCTGCT6140.11165808620949894RNA PCR Primer, Index 38 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)