FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2676304
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT86980.3250004483795563No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT57310.2141386030884384Illumina PCR Primer Index 10 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGT48760.18219155970323253No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTT47170.17625053058247495No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33070.12356593271915298No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT32680.12210869916123131Illumina PCR Primer Index 10 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTTCT27440.10252945853684782RNA PCR Primer, Index 38 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)