FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294523
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT52950.4090309712535042No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT36280.28025767019975695Illumina PCR Primer Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGT28920.2234027514381745No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGTCTT26660.20594458344888428No Hit
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCT19700.15217960592434435Illumina PCR Primer Index 11 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17520.13533942618246259No Hit
ACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGTCTTCT14200.10969291391500961TruSeq Adapter, Index 18 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)