FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020290
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT28880.2830567779748895No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT25860.25345735036117184Illumina PCR Primer Index 11 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGTCTT19500.191122131942879No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGT16560.16230679512687568No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15780.15466190984916053No Hit
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCT13540.132707367513158Illumina PCR Primer Index 11 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGTCTTCT10480.10271589450058316TruSeq Adapter, Index 18 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)