FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031501
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT24530.2378087854495536No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT20120.19505555496310717Illumina PCR Primer Index 11 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGTCTT15360.14890921094599036No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15270.14803669603810368No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGT13160.1275810687532053No Hit
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCT11250.10906436348583279Illumina PCR Primer Index 11 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)