FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005106561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368347
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT11260.30569001512161087Illumina PCR Primer Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT10090.27392648779547546No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGTCTT9030.24514927500427589No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGT6400.1737492093053561No Hit
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCT6030.1637043331423902Illumina PCR Primer Index 11 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGTCTTCT4410.11972406453697193TruSeq Adapter, Index 18 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)