FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1801420
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT68880.38236502314840515No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT51790.2874954202795572Illumina PCR Primer Index 11 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGTCTT40900.2270431104351012No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGT39990.22199154000732754No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30040.16675733587947286No Hit
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCT25610.14216562489591544Illumina PCR Primer Index 11 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCGCGTATGCCGTCTTCT20660.11468730223934452TruSeq Adapter, Index 18 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)