FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3885379
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT126070.32447285065369424No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT85720.22062197793316943RNA PCR Primer, Index 47 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGT60840.1565870408009103No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57420.1477848106967171No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT51250.13190476398827503RNA PCR Primer, Index 47 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGTCTT51090.13149296374948236No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)