FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005106587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215707
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT34390.2828806612119532No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT28410.233691177232672RNA PCR Primer, Index 47 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20180.16599394426453085No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT17260.1419749989100992RNA PCR Primer, Index 47 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGT16640.13687508585539115No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGTCTT16410.13498318262541878No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)