FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180727
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT47020.21561616836953912No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT35020.1605886477307797RNA PCR Primer, Index 47 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28390.1301859425778651No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT23550.10799150925356543RNA PCR Primer, Index 47 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGTCTT23210.1064323961688006No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGT22580.10354345133526573No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)