FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664576
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT17280.2600154083204931No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT15360.23112480739599386RNA PCR Primer, Index 47 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.14806432973805855No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT9250.13918648882896764RNA PCR Primer, Index 47 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGTCTT8000.12037750385208011No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGT7570.1139072130200308No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG7500.11285390986132512RNA PCR Primer, Index 47 (96% over 29bp)
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCT6860.10322370955315871RNA PCR Primer, Index 47 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)