FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1438747
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT72520.5040497043608084No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT58580.40715984116734905RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT36900.2564731672768041RNA PCR Primer, Index 47 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGTCTT36130.25112128817644797No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGT34690.24111257920954832No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25030.17397082322326302No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCTTG24860.1727892395257818RNA PCR Primer, Index 47 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGTCTTCT20750.144222716016089Illumina PCR Primer Index 9 (95% over 22bp)
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCTGCT20730.1440837061693265RNA PCR Primer, Index 47 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)