FastQCFastQC Report
Sun 7 Aug 2022
EGAF00005106599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3729417
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT90920.2437914558763474No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT64300.1724130071804789RNA PCR Primer, Index 47 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47500.12736575180517493No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGT39560.10607556087184672No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT37950.10175853223171342RNA PCR Primer, Index 47 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)