FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786673
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT58960.3299988302280272No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT44930.2514729891815682TruSeq Adapter, Index 7 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTT36970.20692090830275045No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGT34130.19102544226055915No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30280.1694770111822365No Hit
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT26300.1472009707428276RNA PCR Primer, Index 7 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTTCT20110.11255557116495296Illumina PCR Primer Index 7 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)