FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005106609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1678483
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT104190.6207390840419593No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT81700.48674904660934903TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT47290.2817425020092548RNA PCR Primer, Index 7 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35900.21388360799602973No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTT28810.171643084856981No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGT25980.15478262216537195No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCT24750.14745457654322386TruSeq Adapter, Index 7 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20400.12153831763562692No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)