FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2296427
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT65060.2833096806473709No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT52790.2298788509279851TruSeq Adapter, Index 7 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTT44720.1947373027751372No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGT40130.17474973077742076No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33960.1478819052380067No Hit
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT32870.1431354012124052RNA PCR Primer, Index 7 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTTCT25520.1111291584709638Illumina PCR Primer Index 7 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)