FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1753329
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT40260.22962033936585777No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT36420.20771914455301888TruSeq Adapter, Index 7 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTT28880.16471523598822585No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24710.1409319072461586No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGT22830.13020944728570621No Hit
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT22050.1257607670893483RNA PCR Primer, Index 7 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)