FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3421349
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT285570.8346707687523256No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT226510.6620488000493373TruSeq Adapter, Index 7 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTT175050.5116402915925853No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGT154880.45268693722856107No Hit
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCT121950.35643835224059284RNA PCR Primer, Index 7 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTTCT91840.2684321301334649Illumina PCR Primer Index 7 (95% over 21bp)
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCT66210.1935201582767499TruSeq Adapter, Index 7 (96% over 26bp)
CATCTCCGAGCCCACGAGACTAGCGCTCATCGCGTATGCCGTCTTCTGCT47860.1398863430769559Illumina PCR Primer Index 7 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46600.13620358519402725No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)