FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324849
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT22760.1717931628434637No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT19390.14635630173702813Illumina Paired End PCR Primer 2 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16860.12725978583219671No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCGCGTATGCCGT13460.10159648382570391No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)