FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3477734
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT175480.5045814314723323No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT129130.37130499342387885Illumina Paired End PCR Primer 2 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCGCGTATGCCGT93250.26813436565303733No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCGCGTATGCCGTCTT86360.24832261466805686No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT61040.17551658637492115Illumina Paired End PCR Primer 2 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56240.16171449570323665No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCGCGTATGCCGTCTTCT39510.11360845884130298RNA PCR Primer, Index 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)