FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005106655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4018801
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT139390.3468447429967296No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT99360.24723791996667666Illumina Paired End PCR Primer 2 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCGCGTATGCCGT71340.17751563214998703No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCGCGTATGCCGTCTT63890.15897776476118125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50790.126380977809053No Hit
GTATAATAACTGGCCTCTCTTCGGCCCAGGGACACGACTGGAGATTAAAC49800.1239175565050372No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT46700.116203813027816Illumina Paired End PCR Primer 2 (95% over 24bp)
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT41720.10381205737731228No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)