FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533212
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT395697.420875749232951No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT364536.83649280211248RNA PCR Primer, Index 41 (95% over 22bp)
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG266805.003638327719556RNA PCR Primer, Index 41 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT191233.586378401086247RNA PCR Primer, Index 41 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTT119722.245260796831279No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCT113282.12448331995529RNA PCR Primer, Index 41 (96% over 27bp)
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGA112302.106104138691552RNA PCR Primer, Index 41 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGT103291.9371281966647411No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGCTTG82381.5449764821496892Illumina PCR Primer Index 11 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCT60421.133132787709204Illumina PCR Primer Index 11 (95% over 21bp)
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTG44420.8330645221787957TruSeq Adapter, Index 3 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC40620.7617983091153238No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGCT35050.6573370441775503Illumina PCR Primer Index 11 (95% over 24bp)
CTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGCTTGA34780.6522733921967248Illumina PCR Primer Index 11 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCT31510.5909469404289476RNA PCR Primer, Index 41 (95% over 21bp)
ACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC23460.439975094333961RNA PCR Primer, Index 17 (96% over 25bp)
CACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTG15710.2946295282176695RNA PCR Primer, Index 41 (95% over 23bp)
ATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTT15460.2899409615687569TruSeq Adapter, Index 3 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTC12900.2419300390838916No Hit
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCT9310.17460222200550626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7450.1397192861375963No Hit
ACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGC6580.12340307419938035RNA PCR Primer, Index 41 (95% over 24bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCCTAAGA5530.10371109427394733No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)