FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405144
Sequences flagged as poor quality0
Sequence length101
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT344268.497225677783701No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT276456.823499792666311RNA PCR Primer, Index 41 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTT216115.3341527950555845No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGT203365.019449874612484No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG141993.5046798175463536RNA PCR Primer, Index 41 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT132073.2598286041506235RNA PCR Primer, Index 41 (95% over 24bp)
TCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGCTTG114432.8244278577493436Illumina PCR Primer Index 11 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCT106012.6166005173469187Illumina PCR Primer Index 11 (95% over 21bp)
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGA62461.5416740714412653RNA PCR Primer, Index 41 (96% over 29bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCT62261.5367375550421578RNA PCR Primer, Index 41 (96% over 27bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGCT50161.2380783128961554Illumina PCR Primer Index 11 (95% over 24bp)
CTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGCTTGA48861.2059909563019569Illumina PCR Primer Index 11 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC33330.8226704579112611No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTG27080.6684043204391525TruSeq Adapter, Index 3 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTC23910.590160535513299No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTG23820.5879391031337006RNA PCR Primer, Index 41 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCT23610.5827557609146378RNA PCR Primer, Index 41 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCT17450.43071105582212743No Hit
ACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC13330.3290188180005134RNA PCR Primer, Index 17 (96% over 25bp)
ACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGC9120.22510514779930096RNA PCR Primer, Index 41 (95% over 24bp)
ATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTT8400.20733368876251407TruSeq Adapter, Index 3 (96% over 27bp)
ATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGCTT6220.15352566001224255RNA PCR Primer, Index 41 (96% over 26bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCCTAAGA4830.11921687103844558No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)