FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005106679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005106679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196588
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT68763.4976702545424945No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT41522.112031253179238RNA PCR Primer, Index 41 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGT38481.9573931267422222No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTT30211.5367163814678413No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT22521.145542962947891RNA PCR Primer, Index 41 (95% over 24bp)
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTG21121.0743280362992653RNA PCR Primer, Index 41 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCT16660.847457627118644Illumina PCR Primer Index 11 (95% over 21bp)
TCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGCTTG15400.7833641931348811Illumina PCR Primer Index 11 (96% over 26bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCT9880.5025739109203003RNA PCR Primer, Index 41 (96% over 27bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGCT7170.3647221600504609Illumina PCR Primer Index 11 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC5480.27875557002462004No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGCTTGA5290.26909068712230655RNA PCR Primer, Index 41 (96% over 29bp)
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTG4680.2380613262254054TruSeq Adapter, Index 3 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4590.23348322379799377No Hit
CTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTGCTTGA4470.22737908722811156Illumina PCR Primer Index 11 (96% over 26bp)
CACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCTTCTG3800.19329765804626936RNA PCR Primer, Index 41 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCT3720.1892282336663479RNA PCR Primer, Index 41 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTC3570.18159806295399517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2570.1307302582049769No Hit
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGTCT2350.11953934116019288No Hit
ACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCTGC2070.10529635583046777RNA PCR Primer, Index 17 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)